Wide field Microscopy is the most basic microscopy techniques known to the scientists. It is that technique where you can expose the whole subject of interest to the light and the image can be viewed by the observer or a camera. This camera can be connected to the computer monitor. There are a lot of advantages of this particular type of microscopy and they are:
Ultra-fast imaging: This is where a single laser shot is needed to product a one whole image. The frame rate is then rounded to the repetition rate of the laser system or the frame rate of the CCD camera.
It doesn’t let the cells get damaged so easily. If you are observing cells under the WF microscopy technique, then you should know that in the aqueous systems like cells have medium to low repetition rates. These allow the thermal diffusion to occur between the pulses so that the damage threshold is quite high than the repetition rates.
If the subject is large, it can be observed by the WF illumination. They also have the largest penetration depth. They can penetrate deep in to the biological imaging because of the requirement of the large wavelengths. In addition to all this, they have higher resolution that the WF one photon fluorescence microscopy technique. The resolution is better than the multiphoton scanning microscopes.
Ultra-fast imaging: This is where a single laser shot is needed to product a one whole image. The frame rate is then rounded to the repetition rate of the laser system or the frame rate of the CCD camera.
It doesn’t let the cells get damaged so easily. If you are observing cells under the WF microscopy technique, then you should know that in the aqueous systems like cells have medium to low repetition rates. These allow the thermal diffusion to occur between the pulses so that the damage threshold is quite high than the repetition rates.
If the subject is large, it can be observed by the WF illumination. They also have the largest penetration depth. They can penetrate deep in to the biological imaging because of the requirement of the large wavelengths. In addition to all this, they have higher resolution that the WF one photon fluorescence microscopy technique. The resolution is better than the multiphoton scanning microscopes.
